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miR-203a regulates proliferation, migration, and apoptosis by targeting glycogen synthase kinase-3β in human renal cell carcinoma

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Tumor Biology

Abstract

MicroRNAs play a crucial role in cancer progression and metastasis. miR-203a has been identified as a tumor suppressor in various cancers. However, its functions in renal cell carcinoma have not been illustrated. In this study, we detected the miR-203a expression in renal cell carcinoma and evaluated its association with clinical features. Overexpression of miR-203a was found in renal cell carcinoma tissues and renal cell carcinoma cells. High miR-203a expression is correlated with tumor stage and short overall survival time. Bioinformatics and luciferase assay confirmed that glycogen synthase kinase-3β was a target gene of miR-203a. Silencing of miR-203a could inhibit cell proliferation and migration, arrest them in G1 phase, and promote apoptosis in vitro. miR-203a promotes the progression of renal cell carcinoma and predicts a poor prognosis.

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Acknowledgments

The study was supported by the National Natural Science Foundation (81370699).

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Authors and Affiliations

  1. Department of Urology, Shanghai Tenth People’s Hospital, Tongji University, 301 Yanchang Road, Shanghai, 200072, China

    Guanghui Hu, Peng Lai, Min Liu, Liang Xu, Zhuifeng Guo, Huan Liu, Wei Li, Gangchun Wang, Xudong Yao, Junhua Zheng & Yunfei Xu

Authors
  1. Guanghui Hu
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  2. Peng Lai
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  3. Min Liu
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  4. Liang Xu
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  5. Zhuifeng Guo
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  6. Huan Liu
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  7. Wei Li
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  8. Gangchun Wang
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  9. Xudong Yao
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  10. Junhua Zheng
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  11. Yunfei Xu
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Corresponding authors

Correspondence to Junhua Zheng or Yunfei Xu.

Additional information

Guanghui Hu and Peng Lai contributed equally to this work.

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Hu, G., Lai, P., Liu, M. et al. miR-203a regulates proliferation, migration, and apoptosis by targeting glycogen synthase kinase-3β in human renal cell carcinoma. Tumor Biol. 35, 11443–11453 (2014). https://doi.org/10.1007/s13277-014-2476-x

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  • DOI: https://doi.org/10.1007/s13277-014-2476-x

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