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On the problem of linear incorporation of amino acids into cell protein

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  • Physiologica
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Summary

Incorporation of amino acids into mammalian cell protein shows immediate linear kinetics when measured at intervals as small as 5 sec. Free amino acids equilibrate instantaneously across the cell membrane, acting as the precursor supply. Glycine, which does not initially show linear kinetics, may have a significant endogenous precursor supply, but this is not the acid-soluble pool.

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References

  1. D.M. Kipnis, E. Reiss and E. Helmreich, Biochim. biophys. Acta51, 519 (1961).

    Article  CAS  PubMed  Google Scholar 

  2. E. Rosenberg, M. Berman and S. Segal, Biochim. biophys. Acta71, 664 (1963).

    Article  CAS  PubMed  Google Scholar 

  3. W.J. van Venrooij, H. Moonen and L. van Loon-Klaassen, Eur. J. Biochem.50, 297 (1974).

    Article  PubMed  Google Scholar 

  4. R.J. Britten and F.T. McClure, Bact. Rev.26, 292 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. D.N. Wheatley and E. Walker, J. comp. Physiol. B140, 267 (1980).

    Article  CAS  Google Scholar 

  6. J.H. Robertson and D.N. Wheatley, Biochem. J.178, 699 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. D.N. Wheatley, J.H. Robertson and M.R. Giddings, Cytobios24, 75 (1979).

    CAS  PubMed  Google Scholar 

  8. D.N. Wheatley, Cytobios25, 193 (1979).

    CAS  PubMed  Google Scholar 

  9. D.N. Wheatley and J.H. Robertson, Cytobios30, 101 (1980).

    Google Scholar 

  10. D.N. Wheatley, Cytobios31, 117 (1981).

    CAS  PubMed  Google Scholar 

  11. J.H. Robinson, Exp. Cell Res.106, 239 (1977).

    Article  CAS  PubMed  Google Scholar 

  12. W.F. Ward and G.E. Mortimore, J. biol. Chem.253, 3581 (1978).

    Article  CAS  PubMed  Google Scholar 

  13. Y. Hod and A. Hershko, J. biol. Chem.251, 4458 (1976).

    Article  CAS  PubMed  Google Scholar 

  14. G.E. Mortimore, K.H. Woodside and J.E. Henry, J. biol. Chem.247, 2776 (1972).

    Article  CAS  PubMed  Google Scholar 

  15. D.N. Wheatley and M.S. Inglis, Cell Biol. int. Rep.5, 1083 (1981).

    Article  CAS  PubMed  Google Scholar 

  16. D.N. Wheatley and M.S. Inglis, J. theor. Biol.83, 437 (1980).

    Article  CAS  PubMed  Google Scholar 

  17. M.S. Inglis and D.N. Wheatley, Cytobios, in press (1982).

  18. M.S. Inglis and D.N. Wheatley, Cell Biol. int. Rep.4, 295 (1980).

    Article  CAS  PubMed  Google Scholar 

  19. D.N. Wheatley and M.S. Inglis, Cytobios, in press (1982).

  20. E.C. Henshaw, in: Biochemistry of cellular regulation, vol. 1, p. 119. Ed., M.J. Clemens, C.R.C. Press Inc., Boca Raton 1980.

    Google Scholar 

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Authors and Affiliations

  1. Cell Pathology Laboratory, Department of Pathology, University Medical Buildings, Foresterhill, AB92ZD, Aberdeen, Scotland

    D. N. Wheatley

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  1. D. N. Wheatley
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Additional information

Acknowledgments. I thank the University of Aberdeen Medical Faculty Endowment Fund for support, and Mrs J. Robertson for technical assistance.

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Wheatley, D.N. On the problem of linear incorporation of amino acids into cell protein. Experientia 38, 818–820 (1982). https://doi.org/10.1007/BF01972291

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  • DOI: https://doi.org/10.1007/BF01972291

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